FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050220
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGT1900.006070268318.9795976
CTCAGCG10407.711185E-58.3538371
CTCCTGG7950.00425526878.1962181
CCTCTAT16459.2326445E-87.9221791
CCCCATT13652.9053033E-57.42563251
CTTCTGG24507.039489E-107.0922371
CTCAAAG19652.4001565E-77.00050161
CGAAAGT15852.7931812E-56.8517271
CTCAAGG13200.00119827016.5818111
CTCTGAT16655.5395863E-56.52251531
CCCAAAT13550.00161066356.4118011
CTCCCTT14906.779979E-46.3167711
AATTAAC12700.00602709046.24682866
CTTATGT14400.00318902366.03332661
CATCCGT23254.338688E-65.9165521
ATTCAAC15950.00161077385.878326
CTCATTT21053.6219048E-55.8470251
CACCATT23655.792461E-65.8164841
CTTTACT20651.7214187E-45.60967871
CTTGAAT21052.2651454E-45.50308231
Produced by FastQC (version 0.11.5)