FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626283
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATG8354.8219954E-711.2532271
CCCCAAT6355.5723335E-410.2444571
CTTAAAT14801.5643309E-109.7676131
CTTTTAA24650.08.7967961
CTAAAAG10701.1482327E-58.7817241
CTCCGAT12601.0226739E-58.0311491
CTCTGAA13101.7190803E-57.72461651
CTTCACT9850.00355848627.3381061
CCTCAAT11250.00165149937.06741141
CCCGCTT17401.9363451E-67.06187251
CTTCAAC18708.0789505E-76.95746561
CTGTAAG11450.00198943546.94396261
CTCATAT12808.607792E-46.77628141
CTCCAAT13000.00102653886.67203141
CTTCCGT17351.3993307E-56.6656221
CTACAAC13400.00144670336.47286651
TTAAATC14505.126323E-46.4639632
CTGTTAT15751.9383532E-46.42491961
CTATGAT15802.0197187E-46.40458771
CTTTGAT26152.0801963E-86.3573531
Produced by FastQC (version 0.11.5)