FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280996
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAATT5154.2484207E-614.2928691
CTCACTT5559.20558E-613.2627531
CCCTCTA3400.00974066412.9896961
CTCCCGA4600.00542942411.201261
CCACTAT6203.7775122E-410.6850721
CCTATAT8850.00103749638.317321
CTCATAT14851.0428757E-67.93085771
CCCGCTT9300.00169356597.91486841
CTCCAAT11401.847921E-47.74823861
CTTAAAG12456.097727E-57.68600461
CTTCGCT8700.0082325517.61464931
CTTACCG9700.00256181047.58848141
CAAACAT13852.2733549E-46.909081
CAACCAG11950.00257509026.7756571
CTTCAAA26101.9335857E-96.76857661
CGATAAT12200.00320052246.63681131
CAACATT14503.9816558E-46.5993631
CTCAAAG14604.329102E-46.55416151
CCTTCCT12400.00379476776.52976661
CTTTGAC14704.7037855E-46.5095751
Produced by FastQC (version 0.11.5)