FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944082
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAT4300.003468121411.8939081
CCCACCG6957.797444E-611.5638621
CCCGTCT6957.797444E-611.5638621
CTTTGAA13903.8198777E-1110.5126011
CCCGCGT6657.708965E-49.8881681
CTCAAAT6050.00392750239.6611661
CTCCGAT7100.00140359539.2614541
CTATGAT7750.00311068828.4846871
CCCATGA8658.898824E-48.4465411
CTCATAT7850.00349286438.3766011
TTTGAAC9603.076062E-48.2706382
CTTCGAA8000.0041433788.2195411
CTCCGCT8800.009738167.47230961
CTTCAAG11450.00177366417.0191121
CCATAAT11000.0093474486.64205271
CTTTCAG16604.6706282E-56.60204031
CTTCATT12400.00409904776.48135761
CAGAAGA15157.411807E-46.2693961
CTCTTCG13000.0067048246.1822181
CTCTTTT18954.3466112E-56.1688721
Produced by FastQC (version 0.11.5)