FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415635
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTATCGGTCATCTCGTATGCCGT4920.11837309177523549TruSeq Adapter, Index 9 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1900.060.0902067
CACGTCT2650.043.083549
ACACGTC2900.039.3694468
AGAGCAC3150.038.5055543
GAGCACA3350.038.3411374
AGCACAC3400.035.678565
GCACACG4050.028.1904646
ACGTGAG1050.00832429127.1836646
GAAGAGC5050.025.498651
AAGAGCA5700.021.2845082
GTCACTA1701.8189894E-1215.11091820-24
AGTCACT2054.4383341E-1011.8348420-24
ACGTCTG2353.092282E-1111.53748710-14
CTCGTAT2703.6379788E-1211.09999735-39
ACTATCG2509.458745E-1110.84549925-29
CCAGTCA2302.9904186E-910.54844420-24
CAGTCAC2501.0786607E-910.27542520-24
CGTCTGA2951.8189894E-1110.15834110-14
TCGTATG2703.8380676E-1010.04285340-44
ACTCCAG2807.428571E-88.66395915-19
Produced by FastQC (version 0.11.5)