FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793593
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2452.2317708E-623.2550669
ACACGTC2502.657087E-622.7928358
CACACGT2650.002315522616.1270077
AGAGCAC4302.790296E-413.2524843
AGCACAC4655.400449E-412.2549865
GGATTCA7450.0027322278.6068472
CAACAAG11051.8594728E-58.4505441
TCAGTTC10600.0083494546.720013
CTTTAAT12000.00347387816.58438251
CTTTGTT12300.0044971816.4237881
CAAGAAT14050.00263979436.1349151
CAAAGAT15800.00150654295.91003271
CTTTCAT14650.0042107825.88365551
GATTTCA14700.00479906945.8148655
Produced by FastQC (version 0.11.5)