FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916026
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGC253.843278E-4108.90866145
CTTAAGA3050.00494651614.3895031
CTTATGG5851.6871769E-512.5036981
CCTCATT6900.00106972729.5408661
CACCCAC6250.00508739479.3627691
CATTGAG6700.0090036488.7339271
CCCTCGG7650.0027369078.6054861
CTTCTGT12155.34E-68.4284181
ATTAAAT12407.021181E-68.25849151
CCCGCTT10709.514386E-58.20336151
CTTCCGA8050.00433723548.1778851
CTCATTT9000.00130303278.1274041
CTTCTTT23403.6379788E-128.1274041
CCTGTAT8550.0074505837.69964551
CTTCGAT10607.7214616E-47.59068871
CCCTTTT12053.7622033E-47.28431221
CTTCAAG12204.3360607E-47.19475131
TAATCTT11050.0013746197.19014369
CTTGATA11250.0014540347.1521161
CTTCTGA10250.0046652717.1362571
Produced by FastQC (version 0.11.5)