FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110680
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAGT6450.0069441919.01545051
ACTGTAC6500.0077633038.8937446
GGGGGGG1550.00338703588.406665120-124
GTTTAAT8600.0083073137.6067861
CCCAAAG12759.57504E-57.41123061
CTTTGAT19702.3346729E-77.01042751
ATTTAAT16053.4103836E-56.7534276
ATAGCCG11850.00286152266.70783427
CCTTGAT14253.7542026E-46.6311011
TTTGATA22551.04441046E-45.45208262
CTTTCAT23258.725794E-45.00212051