FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459654
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAC2500.001337397417.503871
TCAAAAC5401.2535758E-412.0298512
CTCAAAA5852.3959875E-411.2204281
CTCCAGA4600.005805569711.0984681
CTGCAAT7351.4756119E-510.9151111
CTTCACA4900.00928121110.418971
CATCTTT8300.00642295237.826653
CAAAAAG10600.0066337796.88045171