FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200658
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT9250.014.4763087
CACGTCT10950.014.1597139
ACACGTC10658.185452E-1111.9115648
GAGCACA13706.486516E-99.2605614
CTTTAGA9051.9899887E-48.6120231
GCACACG18803.110472E-108.2472796
AGCACAC18752.561137E-97.89339645
AGAGCAC16351.3091812E-77.75961453
CACCCAT13950.0028468846.09492161
CCCTCAT14300.00375464745.9457451
CCCCCCC3050.0039297315.9220066115-119
GAAGAGC24051.1061018E-55.59757141
Produced by FastQC (version 0.11.5)