FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513661
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCCA4404.0362732E-412.6842364
GTCAAAT5201.0590705E-412.2497191
CTCAAAA7603.3541938E-49.3126511
AACAATT6900.00164430649.1004462
CAACAAT7150.0019954128.9088861
CCATGAT6350.00854103558.7890763
CTTCATT7350.00256324988.6664681
CATCAAA11001.9178337E-47.7210341
Produced by FastQC (version 0.11.5)