FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949474
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAT1658.450153E-526.3489111
CTCTACT3350.00979728512.9778211
CTCGATA4300.003689962411.7957341
CTTGAAC7151.435483E-410.1341971
CTTTAAG7301.7701185E-49.925961
CAATTAC7150.00161383679.1207771
CTCCAAT8004.4389386E-49.0574381
ATCAACT7200.00171958259.0574381
CTAAAAG6700.0097269558.6518811
CTCAAAG8659.661737E-48.3768211
CCTCCAT11854.005E-57.9491441
GGTAAAT11552.575488E-47.52826071
CCCATTT11050.00132892447.2131641
CTTAAAT10150.0046491777.13886741
CTTAAAA12305.3052034E-47.06922051
CTCAAAA11500.00202710956.9309091
CTTTGAA15301.2851524E-46.6302821
AAACATT16705.9154627E-56.4905092
CAAAAGG13400.00140502516.48891071
TCAAAGG12300.00422686346.4623662