FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439418
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT10400.00603303176.9504292
CTTTAAT18202.7448532E-56.3668451
CTCAAAA20503.5445028E-66.359081
CTTTAAA22854.7806134E-76.3389811
TGTAAAC12950.00722080336.1387137
ACTCTTT19250.00250705155.25705153
CAAAGGT18300.0079525735.13496163
TTCAATT21200.00150233065.1144662
CGAAAAG21350.00160827385.08825731
AGATTTC18650.0099195495.03841974