FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288980
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT4650.027.597859
CACACGT4200.027.1597887
ACACGTC5750.024.7980678
AGCACAC8704.2564352E-1013.1151855
GCACACG8807.07405E-912.1557646
AGAGCAC10105.4073098E-810.591163
GAGCACA10651.1751399E-710.0441994
GAAGAGC19002.1649612E-87.51021341
AAGAGCA19201.5553815E-66.6864482
GTCACTC4503.23522E-66.0204220-24
AGTCACT4751.2715136E-66.003742720-24
ACGTCTG6157.3184856E-75.33259310-14
CACTCAG5756.569746E-65.207594425-29
ACTCAGA6053.0114843E-65.1850525-29
CTCAGAC5807.688326E-65.162701625-29
Produced by FastQC (version 0.11.5)