FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905866
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAG8255.6887184E-59.6594831
GGGGGGG1953.0656363E-48.274912120-124
CTTTACG9350.00208657377.74824761
CTTTGAA14558.3003815E-67.4686721
CAACAAA15751.8779941E-46.4396551
CAATAAA15200.00576820225.7194311
CTTTTAT17809.0522924E-45.69800951
ATCAAAA23752.2124854E-45.18561651
CAAAGAA21050.00132490255.16243121