FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987712
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATT6153.5158015E-410.76787951
CTCAGCG6859.482055E-49.6675121
CTATAAA7350.00180410779.0098581
CTCAAAA13101.5535079E-78.9869321
CTTCTAT8355.839594E-48.81203651
CTCATGG9401.9908242E-48.6104851
CTCCTCG8450.0063602577.8369771
CACCAAG10607.25026E-47.63571361
CTCAAAG9700.00257000987.585621
CATCATA13752.5772328E-57.4918331
CCTCCAT9850.0029868117.47010231
CTCCTGT10150.00400374137.24931141
CCTTCAT12404.88286E-47.12069461
CTTTTAA13651.9089876E-47.00766751
AGAGAAT10650.0063887176.90896751
CTTTCAT14152.9665278E-46.76004651
CCACGAT12650.00469032076.39830541
CATCAAT18551.9557797E-45.94990631
ATCAGAT15200.00485633035.80898761
GATATTG15400.00692867955.62554655
Produced by FastQC (version 0.11.5)