FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157476
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT6400.00775561958.894871
ATAGACA8900.0092208738.708399145
CTTTAAT10501.0534411E-48.1324521
CCTCATT8850.00144963048.0405611
TACGATC8200.006791447.7800852
TTAATAA9200.00221814387.70058873
GGATCGT9550.0030628387.45119951
CCAAAAA13603.4738812E-57.3251871
CATAGAT9900.00435198667.18777371
CAAGGAT14751.01014084E-46.754071
CTTCGGT10550.0080629486.74492451
CTTCAAG13050.0091736745.9980731
GTTCCAT15950.00188917335.799791
CTTCCAT19757.1913924E-45.4044781
TGTCCGG17250.00503533575.33907465
CTTTTGT17650.00625662655.24116951