FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000192
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAGT4000.002155124412.6733461
CTCATAT9251.7543516E-610.1778211
TTGCAAC9300.00205281337.76130729
CCTCAAT8400.00695532567.7591911
CTCAAGG10306.323474E-47.734081
CTTCGAT11502.4659684E-47.55677751
CTTCTGT13301.6869736E-47.07856041
CTTCAAA15702.431167E-56.9190241
AGATTGT11500.00210835766.90522536
CTTTTGA14404.4593477E-46.5378371
CTTCAAT14605.2729377E-46.44827751
AGGATTT16303.0665792E-46.20045333
CTTTGAT15359.6622756E-46.13321541
CTTTTAT18400.00138547695.510151
GAAGATA17100.00363784075.48820352