FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478402
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCACACATGATCTCGTATGCCGT9960.20819310956057877TruSeq Adapter, Index 7 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2500.064.190917
CACGTCT2850.056.307829
ACACGTC2900.055.336998
AGCACAC3700.043.3722345
GAGCACA4250.039.4010734
GCACACG4350.036.891336
AGAGCAC4950.032.4196553
CTTAGTT1050.00916648626.6525141
AAGAGCA6550.026.6419532
GAAGAGC7300.025.876671
ACCACAC3200.010.90312925-29
CACACAT3200.010.46700425-29
ACACATG3200.010.4659130-34
CCACACA3100.010.35445525-29
CAGTCAC3600.09.69106320-24
AGTCACC3353.6379788E-129.58113320-24
ACGTCTG3457.2759576E-129.30303110-14
GGCAAAT6150.0064155959.1008591
CCAGAAT6150.0064155959.1008591
CTCGTAT3753.6379788E-128.9309135-39
Produced by FastQC (version 0.11.5)