FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619750
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC8750.016.194828
CACACGT8750.016.194827
CACGTCT9950.014.9537619
GCACACG12250.012.1486666
AGCACAC16151.1859811E-98.7764615
AGAGCAC19153.4688128E-97.7716373
CTCGAAT11000.00156473077.10394531
GAGCACA21752.908473E-76.51677474
CCCACAT16456.0965933E-56.4777611
CTACTGG12400.0055028296.30188661
CCCAATT12650.0067710836.1773441
CTCACAT12900.0082927436.05762771
CTCATAT23255.987087E-65.8053741
CCCTGAA20802.5051867E-45.4645731
TTTCGAA20109.611975E-45.28940632
GTTCTGT28706.781529E-65.1980091
Produced by FastQC (version 0.11.5)