FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459438
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAT13905.0387825E-88.8746461
CCCGAGC7800.00351181168.3726871
CTCCAGG10004.5160763E-47.98196131
CTCTGAC13051.3056875E-47.22852561
CTCGAAG11350.00174101077.03256461
CCCAGGA10400.0058154086.97723871
CTCACGG13300.00127256096.5470631
CTCAGAT12350.004228296.4631261
CCACCGA15550.00110451986.06638341
CTTTTAT61800.05.75341
CCCTGAT17450.0043562165.40586041
CACATCT17900.00622865.2438153
GGCAAGT18100.00669259675.21172761
CTAAAAG19900.0036218615.10495471
GTTTTAG21400.0016187295.08621171
ACGATTT31304.510299E-65.0749923
Produced by FastQC (version 0.11.5)