FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161005
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATT28900.07.7546351
CTCAGAG12104.525418E-47.16957431
CCCAAAT29109.458745E-116.7076171
CTCAAAT35809.094947E-126.26002651
CCCAATT22552.6494235E-66.0912241
CCCCAAT21851.03115E-55.95550441
CCCAGAT34801.1041266E-95.81669471
CCCCGAT21957.0118214E-55.5990181
CCCACAT26654.3815504E-55.15411231
CCTTGAT53805.456968E-125.10621171
Produced by FastQC (version 0.11.5)