FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856314
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTG4200.002928777412.1652881
CTCCCTT6152.8786471E-511.8685721
CTCCCAA4650.006261723610.9880021
CCCAAGG4700.006779998510.8711081
CCCAAAG6153.7857372E-410.6817151
CTCAAAA12306.200935E-910.0882861
CCTCAAT6150.00453205179.4948581
CTCTTGA6450.00670706679.0532371
TATGTGC6400.0069427499.0152587
CTCATCT6600.0080990018.8474821
CTTATAT11954.392761E-68.55133151
CTCAAAT8750.0010067358.3419111
CTTTAAG9250.00174326457.8909971
CTTAAAG9350.00193789017.80660151
CCTATTT8550.00759223247.68333961
CTCATTT9950.00356492267.33585171
CTTTATC16606.272312E-67.03534751
CTTAAAA13752.3030213E-46.90103631
CTTTGCG13350.0012394236.5610541
CTTGAAG12650.00509820276.34710651
Produced by FastQC (version 0.11.5)