FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587913
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT16106.978189E-88.0550431
GTTGCTA9650.00300660337.46610261
CTTCAGT14808.9926056E-56.8153411
TCACAGT13200.00131040736.53015952
AACGTAA13700.00203248166.28089145
CTTAAAT21901.7015918E-66.250731
CCCCAGA13300.0098275575.95884751
GTTCCGT26109.1467155E-75.79695651
GTAGAAT15250.0063705135.66934251
CTTTGAT40001.9099389E-105.58371161
GTTTTAC26959.82269E-65.3467821
CTCAAAT20258.5292687E-45.33688071
CTCCAAT20500.00100494925.27179671
CTAAAAA29307.64387E-65.16384171
CTAAAAT22606.64908E-45.10073571
CGTCAAT27005.74167E-55.07003641
Produced by FastQC (version 0.11.5)