FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086127
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC6850.00122386549.4026139
CACTGGT13803.8529462E-57.26886031
CTCAACC12250.00443247666.4338951
GTCACAT14500.00386939275.9296721
AAGATCT16050.00189681475.79843333
GTCCACG15350.00727732955.60131841
CTCAAAC15400.0076153055.57831342
CCCGCAA19857.028209E-45.41437241
CAAAGAA50505.456968E-125.24961851
CCCAGAT20600.0011540725.2172471
GGAGAGG18250.0086623055.09773358