FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3978868
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAA26901.8189894E-127.58983231
CCCAAAC24504.5274646E-96.84523251
CTCCGAT33351.8189894E-126.7778551
CTCAAAA54100.06.60427671
CTTAAGG14404.1132397E-46.5827491
CCCTGAT31356.91216E-116.5124881
CCCAAAA38201.8189894E-126.29907851
CTCACAT17751.21491474E-46.1619671
CTCGCAT13300.0086863646.03069641
CCCCATT25705.49624E-75.9581661
CTCGTGA14700.00371038055.9523761
CTACGAA22151.1186648E-55.92550231
CTTTAGA23404.295689E-65.92057851
CACTACC20753.0430889E-55.91450936
CTCTCGT16250.00176700335.83332821
CTTTTAT84100.05.8090521
CCCGGTT31452.060915E-85.7962321
CTCACGG25402.8106333E-65.7414651
CCCAGAA31852.7164788E-85.72343831
CCCAATT22951.9796747E-55.718951
Produced by FastQC (version 0.11.5)