FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707940
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGT1100.00971614226.3377251
CCTTAGG1750.0037471420.6280784
TCTATAC3355.776051E-415.0862077
CACAGTT4401.7765278E-514.814971
CCTCAAT5601.1907823E-512.9337031
CTCCAAA7501.5795686E-611.5885981
CTCCAAT5100.001019376211.3613711
CTTAAAT6355.459882E-410.26549151
CTCCATT7753.241389E-49.3456441
TTAATTC8000.004609428.1223063
GTTTTAA8050.00473739638.0976241
ATTTGAT12255.082856E-47.0950611
CTTCCGG13702.4197382E-46.87280041
CATAATC10900.0093031446.6448391
CTTTTAT14856.458518E-46.3405641
CTTTTCG12750.0059943996.24875451
TTTTTAC13300.0095950975.9712866
CTTTCTT17800.00560574055.289741
Produced by FastQC (version 0.11.5)