FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1628439
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT7651.6039849E-812.8652989
AGCACAC10002.7284841E-1112.6539395
GAGCACA9501.6007107E-1012.5807134
ACACGTC8404.3874024E-912.5535128
AGAGCAC9502.1445885E-911.8406723
GCACACG9753.2287062E-911.5363556
CACACGT9101.7879029E-710.8153337
CAAACGT12651.2250907E-47.26551961
GTCAAAA15101.9763118E-57.02308851
CCTCGAA12800.00110651196.62804031
AAGAGCA18851.0384598E-56.34158042
CCCGAAA22201.1627982E-45.41389471
GAAGAGC20109.932561E-45.2760521
GTTTGAT17900.0079615015.1345711
Produced by FastQC (version 0.11.5)