FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482969
Sequences flagged as poor quality0
Sequence length100-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACAAT3303.4185345E-516.9525741
GTTCTAC3150.008501491513.2578185
GTTTCAA4100.003366898711.9391591
CCTCCAT5850.0042667489.562991
TCCATCG6250.0076401638.9092543
GGCAGCC10950.00184271816.99213657