FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096006
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAG10805.456968E-1212.7216171
CTCAGTT5400.001666592910.712941
CTCAATT7803.1368894E-510.1978951
CTCCGAC6150.00489720669.4064851
CTCAAAT9251.9712856E-59.3810611
CCCTTGC9302.1009544E-59.3306261
AAAGGGT7300.00205096238.8838963
CACGATT7350.00218668568.8214499
CTCGCCG7700.00322176568.4520921
CTTTATT24701.8189894E-127.9045881
CCATGAT14155.748503E-67.66561941
CTCAAAA15302.1352298E-67.5620751
CCCATAT9800.00336920827.3788111
CTCCGAA9850.00354090577.3413551
CTCCGAT10200.0049755827.0894451
CCCAAAG10700.0079109236.75816351
CAATTTA10800.0089529356.67206244
CTTTCAT22002.4735527E-76.57384971
TTAAAGT12350.00452527786.42048932
CATTGAT20303.091809E-66.41193251
Produced by FastQC (version 0.11.5)