FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194867
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAGT7400.00226441868.7877056
CTCCGAT11050.00129549477.2308781
CTTTAAA15801.8929728E-46.43623071
CGAAGAT13800.00190546286.31630041
CTGAAAC15409.695576E-46.1317331
TTAAATT15650.00124569526.0052352
CCCGGAT15000.0048402655.8109961
AAATACG16650.00262641775.641496
GTTTGAA16000.0098715285.44780971
Produced by FastQC (version 0.11.5)