FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2898813
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAG1600.001989131823.0091571
CTCAGGG10550.00580121956.9790811
CTCACAG15551.2919083E-46.6290061
CCCAAAG18905.300837E-66.62274171
CTTCGAT29656.730261E-106.45653251
CTCGGAT12950.0059553526.2542261
CCCAATT13100.00671194176.18261341
CTTAAAA42550.06.05646471
CCCGTTT24901.6605445E-65.9140011
CTCCCGT20251.0205995E-45.81762461
CTTTGAA44409.094947E-125.638281
CTCAATG25001.112672E-55.5958271
CCCACAT19854.87372E-45.56392771
CACCAAC22507.724897E-55.5631031
CAACCAT24003.4623692E-55.5221981
GTTTAAA25701.7670807E-55.4434121
CTCCAAG18950.00163406415.4396321
CACCAAT24404.503612E-55.431671
CTTAAAT28753.6446763E-65.3781411
CTCCGAC19250.00199152955.3548591
Produced by FastQC (version 0.11.5)