FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546985
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGA1952.589679E-422.3367961
CTCAAGG3456.922535E-414.7293371
CACCTAT4252.1506107E-413.6648631
CTCGTCT3200.00714482113.6114851
CCTCGTT4350.00396365911.6818881
CTTAAAG5109.994056E-411.3873851
CCCAAAA5600.002180676210.3706551
CTCCTTT10508.1568214E-79.6792781
AAACTTA6150.00487771029.40954
CTTCTGT7300.00191405228.9500171
ATCAATT7450.00237451668.740122
CTTCCAT8950.00132699998.1111261
TCCATTT8450.00738822957.7057862
CATCAAT8500.0075558167.68648531
CTCAAAA8750.0097885677.46687171
CTTTCAT10450.0060488776.94685031
TCCTTTT12909.270526E-46.7301182
CTTTTAT12100.00338668216.59950731
CCTTTTT15952.249925E-46.34920023
CCCCCCC3050.00614827555.6876044100-104
Produced by FastQC (version 0.11.5)