FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457041
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACT5400.001431800210.9102341
CTCACAG8405.796906E-59.64386751
CTCTAAA8607.4992364E-59.4195921
GTTAAGC6600.0087980478.7592638
CTCCGTG8759.226927E-48.4164671
CCCAAAT8950.00115426018.2283891
CTCAAAT14657.265984E-67.54034951
CTCGAAA8850.0095499137.48922821
CTTAAAA20858.540155E-97.41738941
CAAAGAA43600.07.26306341
CTCAATT10700.00663407856.88262461
CCAAGCA10550.0069498156.84966048
CTCAAAG14805.07915E-46.46873661
CATTAAT17408.041292E-56.3486281
ACCTTTC16653.9846005E-46.07624533
CTTCAAT24651.3788213E-65.97517871
CTTCCAT29753.1453965E-85.94103531
CTTCATT27552.668039E-75.8808341
CTTTAAA25302.1907726E-65.82166621
CAACATC20451.5366348E-45.6539014
Produced by FastQC (version 0.11.5)