FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470125
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC6353.400879E-612.4577598
CACACGT5401.3008539E-411.9858747
AGCACAC6857.919818E-611.5488275
CACGTCT7803.3350712E-510.1418939
AGAGCAC8105.039305E-59.7679293
GAGCACA8508.539709E-59.3082624
TAACGCT7050.00152143649.1806699
CTTTAAA12206.6210832E-68.2957381
CACATTT10004.959984E-47.9120223
TCACATT9200.00190098937.82272772
GCACACG10550.00728277166.8166116
CCCGAAA14153.685145E-46.6416161
CAAAAAT20201.2806636E-45.72605371
CCCAGAT15500.0073389875.5967561
GCAAATC19404.858896E-45.5646212
Produced by FastQC (version 0.11.5)