FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990106
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTA5150.001085237511.277521
GTCTCGC4600.00601560411.0476781
GTCTTGG5950.0036072349.7612151
GTCAAAT9703.119916E-69.7297681
GTATGAA9853.7942536E-69.5815991
TCGAAGA10055.186457E-69.3491572
GTCAAGA9101.6201787E-48.7757071
GTCCATG9552.73187E-48.3621931
CTCCGGG9703.2319705E-48.232881
CTTCGGT9803.6094806E-48.1488711
CTCAAGG8150.0051868338.0170711
GTTTCAA11853.910506E-57.96445131
CTCGAAG8400.00680229867.77846861
CTCTTCT12658.813499E-57.460771
GTATAAT11050.00130221697.22705361
GTTATAA10450.0060555976.9472761
TCAAATT15702.499146E-56.90536742
GTTTTTA14103.3475342E-46.69352871
AATTTCC14454.8053658E-46.49775845
GTCTTTT15952.1530782E-46.3723291
Produced by FastQC (version 0.11.5)