FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3075367
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAC15704.5352535E-67.19233271
CCCCAGG11550.00280537626.7214061
CCCGAAT16657.8906385E-56.3580871
CCCTGAT26604.8248694E-86.10232931
TTGGATC12800.0083570326.05318559
CTTCGAT29409.602445E-96.00125551
CTCCGAA23551.3087538E-65.99361041
CTCCGTT24302.2860004E-65.80862241
GTTCTGA26853.711557E-75.7826621
CTCACGC16000.00216720145.73419951
CCCACGG20051.6278138E-45.6319011
CTTTGAT47650.05.62820771
CCCGGTT23053.5739904E-55.51126151
CTCAAAG31301.2701439E-65.18600461
CCTCGAT26305.2368636E-55.0985571
CCCAGAC18150.0095597775.0549421
CCCAAAA25401.6527346E-45.00136141
Produced by FastQC (version 0.11.5)