FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646034
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGG4252.0999012E-413.7040681
CTCGATC4300.003875387611.7176922
CCTCGAT6355.2053947E-410.3185161
CCAGTTT7301.8905096E-49.8602893
CAGCAAC6500.00729543258.9603521
CTCATCC8050.0045215168.1394511
CTTTGAA11001.3843391E-47.94213061
CAGATTT11151.8478592E-47.7467383
GGCAGAT9450.00220518687.70400671
AAATTTC10306.738956E-47.68719634
CTGATTT10759.4204966E-47.4495961
GCAGATT9850.0036987717.3076252
CTTTGAT10200.0046526617.13753561
CAAAGAA16103.2002783E-56.7828761
CAAAAAC10900.0088497366.6791621
CAAAAAA19501.0196278E-56.3469161
CTGAAAA16750.00257184295.6503711
Produced by FastQC (version 0.11.5)