FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334597
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGT5450.001643230710.731411
CTTAGAA6500.0070578288.9978741
CCCTTGT10809.48633E-47.44615751
GTTTAAT9850.00318303177.42210441
CTCAAAG14901.0227266E-57.3598391
CTCAAAA17251.4108227E-67.20481971
AACGAGC10200.0050210027.08307366
CTCATAT12000.00289087286.7015421
CTTTAAA18002.0324047E-56.49846461
CATCAAT20402.804276E-66.4506821
TCAAAAC15701.8746455E-46.44123132
ATATAAC14655.661655E-46.4110276
CAAGTAT12800.0064182096.20875643
CTTCAAG17801.21552635E-46.16076331
CTTTGTT16904.1558774E-46.0562621
CTTCCTT23303.807827E-65.96157461
CAATGAT16000.00142035455.9400031
CTCCTTT26157.20358E-75.87098461
ATCCATT14950.0043421575.86817931
ATTTCAG18903.2385814E-45.73391636
Produced by FastQC (version 0.11.5)