FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763716
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATA5008.3533715E-411.6348261
CCCAAAT5950.00355726719.77716351
CTCAAAG7702.9193424E-49.4438511
CTTCTGA8851.17493895E-49.0383531
CTCCACG7250.00177221279.02701951
CTATGAT7650.00288552678.5550191
CTTCGGA8800.00110530928.263371
CTTCACG9100.0015394157.9909521
CTTCAAG9150.00162487767.94728571
CTTTTGT14406.793418E-67.5747561
CTTTTAA10750.0078339016.7644341
CATAAAT12000.00305366216.66578531
CTTTGAT13350.00129282086.53641841
TATGATT12300.00417554056.4695412
TGATTTC15801.9975613E-46.4087234
ATCAAAG12700.00552234756.298381
CTTCAAT13200.0082436486.0598051
TTTATCC15550.00114529596.04663133
CTTTCAA16200.00175363175.83536771
CTTTTAT16650.00243068845.67765471
Produced by FastQC (version 0.11.5)