FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518262
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4801.4303623E-716.493147
CACGTCT5209.0815825E-512.4563589
ACACGTC6250.0058176859.2121698
AGCACAC9151.8912801E-48.6521395
CGCATAA10900.00116736357.30247451
CTCCTGA12054.264248E-47.2060631
CCCCCCC2150.00838621156.71856520-24
CTTGGAA15751.9102078E-46.43207841
CCCGCCT12500.00493471376.3677581
CGTTATT12500.00522119136.3333664
CTTTAAT21709.063262E-66.00228551
CAAGAAC17708.567328E-45.72345921
CTCAAAA20251.3089555E-45.7174031
GTTCATT15400.0067612085.63851071
CCTCCTT19200.00239496845.27631431
AATGGAT19850.00387817575.0766433
Produced by FastQC (version 0.11.5)