FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693610
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAA3609.136456E-414.2002241
CTCGTTT5551.4528561E-411.8426581
CCCACGG5400.001533676210.8192181
CTTGAGG5500.00178707710.6225051
CTCATCT4900.00919830910.4328171
CCTGTAT7351.7505372E-49.9360161
CTTATAT8456.7663044E-59.5068281
CTTTAAA8356.2876684E-48.7460741
AAATTTA11502.8684904E-58.163254
CAAATTT10851.297665E-47.9867313
CCTTGAT10607.847362E-47.5785561
CTTTAAT8750.0092865817.51162861
CTTCATT10950.0089776476.6693811
CTTCTTT18652.3273904E-45.8737041
CAAGAAA16000.009288965.4772291
Produced by FastQC (version 0.11.5)