FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712055
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAT7156.146547E-813.1809341
CTCCCGA4050.002346127312.5300241
CTTACAA6303.9465885E-511.5071651
CTCAAAA10153.7271093E-911.4278051
CTCAGCA4900.0097132610.3564481
CTCTTGT5650.00237622310.2647971
CACCAAA6456.251463E-410.11561
AATACGC6050.00429754479.5558177
CTTAAAC6100.0044808479.5075591
ATTTCGA6400.00665242979.0618921
TCCAATT7250.00187052058.9734572
CTCAAAT7650.0029661978.5288391
CTTTGAT11603.045159E-58.1244551
CATTAAT11151.7021438E-47.80216741
CTTATAA13352.1181706E-57.60248661
ATTTCTC11903.7411036E-47.28731446
CAATATT17602.314362E-66.9802254
CTTCATA11600.0022074586.8745391
AGATTTC10900.0095073996.62989574
TTCTATT14555.1649916E-46.458562
Produced by FastQC (version 0.11.5)