FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081701
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAACC5100.001087214911.2752522
CCCAAAC6505.3685704E-511.1663061
TTAACCG4800.00888593610.4824623
CTCCAAT9051.2882465E-610.4259981
CCTCGTT7101.3151285E-410.2226741
CCTCGAT11452.4059773E-810.1423221
CCAAACC6250.0058757949.2006062
CTACGAT7500.00245474028.7097191
TCCAATC10156.30422E-58.4980972
CTTCGGA12004.5906967E-57.86294031
CTTCGAC8550.0079886837.64010431
CTTCAAT16801.0405147E-67.34450441
TCGATTT11803.6161678E-47.3098043
CTTTAAT14254.953403E-57.13076351
ATATAGT10700.0083900736.71711166
CTTCATT16253.810208E-56.6997841
CATCACC17451.0210784E-46.23905371
CTTTAAA13050.00747469866.11793761
CTATTTT19055.1705552E-56.09604071
GATTTCA19801.0426002E-45.80792675
Produced by FastQC (version 0.11.5)