FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205830
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAA5400.001629649310.7419571
AACACTC5400.001677215610.7049412
CCCCAAT6900.00115960559.4575921
GAGTTGC6550.0082957338.8221526
CTTCATT16106.890332E-98.55686951
GGGGGGG1600.0043782628.170857125-129
CTCTTAT8550.00806206657.63244341
AAGTGCG8700.0098157717.46601349
CTTCGCT9800.00328189627.3987971
CTCCAAG10950.00119880187.283931
CTTCAAT16606.9358703E-66.9887431
CTCCAAA12506.334248E-46.9607881
GATATTG24152.9354851E-86.5800835
TTTCGAT16854.647394E-46.0036612
GTTCCGT13300.009192675.9969191
CTTAAAA13400.0099315125.9521661
CCTCGAT14700.00394367335.91903731
CAAAGAT19500.0028334045.2057171
CTTTGAT19550.0029256085.1924041
CATCAAG20200.0043977235.02532151
Produced by FastQC (version 0.11.5)