FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515085
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGATCTCGTATGCCGT6560.12735762058689343TruSeq Adapter, Index 6 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3250.030.1499948
CACGTCT3250.030.1499949
CACACGT3350.029.2499927
GCACACG4209.094947E-1221.7749946
AGAGCAC4552.910383E-1120.1019483
AGCACAC4603.274181E-1119.8815175
GAGCACA5201.9099389E-1017.5909124
ATAGCGT2600.003627781315.0749968
AAGAGCA7951.8189894E-1214.7963092
TTTTAAC2700.004662239514.5166626
GAAGAGC8151.0327676E-711.24877551
ACATATC4350.0086792210.5141084
CTTTTCT8150.00145644948.034841
GGAAGAT7900.0098650497.46019551
AGTCACT3203.2661774E-77.34763420-24
AAAAAAA12050.07.15579260-64
GTCACTC3005.4404805E-56.54839371
CAGTCAC3602.2935728E-66.5312320-24
ACGTCTG3805.5442997E-66.18760110-14
TCACTCC3908.452773E-66.028827725-29
Produced by FastQC (version 0.11.5)