FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541767
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT23753.6379788E-128.0039371
CCTTTAT23402.5465852E-117.81120541
CTCAATT12951.08080356E-47.33951761
GGTAAAC13101.2460147E-47.2554771
ATCACAT17401.6163958E-67.14321231
CATTGAT29952.0008883E-116.8352621
CCTTGAT24705.0476956E-96.80808261
CTCAAAA23551.0077565E-76.51962041
ATCGGTT15107.072154E-46.29448651
CTTGAAT21054.677664E-66.25193261
CTCATTT21104.8619204E-66.2371181
CTTGATT24301.2159835E-66.0175211
CTTGCAT15850.00126803195.996641
CTCCAAT14650.00346260565.98876861
TTTTACG17206.164251E-45.87306266
CTATAAT20351.2109026E-45.7484331
CTTTTAT39509.276846E-115.7379731
CTCATCG19203.4129518E-45.71194461
CTAATAA15550.00671324655.64215141
CTCTTTT38953.8198777E-105.63128761
Produced by FastQC (version 0.11.5)