FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459602
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCAC8909.4613824E-710.6809571
CTCACAA11853.840296E-68.6390591
CCTCTGG13151.7301481E-68.341081
CTCTTAT17753.8799044E-98.2392641
CTTAAGA10456.66519E-47.6972071
CCTAATT8800.0096772127.47853571
CTCAGCT13802.944379E-57.41832261
CTTTAAT31900.07.335271
CTCCGAG14103.9131242E-57.2604861
CCCACAA17301.4746911E-67.18554261
CTCCCTT18706.6749453E-77.0386231
CCCACGA13601.9741766E-46.9897431
CTTCAAT37800.06.96413951
CTCATCT19201.0331732E-66.85532471
CTTATTT37400.06.84310531
CTCCAGG19251.0785334E-66.8375191
CCCCCAT10850.0081335646.73949051
CTCAATT15201.04992534E-46.73505541
CCTACAT14253.495775E-46.67091271
CTCATGA14353.8068887E-46.62442541
Produced by FastQC (version 0.11.5)