FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234265
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAC6506.977048E-49.9966921
CCCAACG8407.199461E-59.4545431
CTCATTT10004.938538E-69.3857821
CTCTCGT6650.0094265248.68551351
CTTCGGT12851.4889592E-68.427821
CTTCTAT11151.9558716E-58.4177431
CCTCATT9452.589875E-48.4040381
CTTTTAT18651.4370016E-98.129571
AATAGCA8100.0053322767.9927627
CTCATAA10055.024075E-47.90230461
CTCCTGT11051.6109804E-47.84054231
CTCCGAT9250.00194278987.80522441
CTTTAAG8350.0067782147.7818561
ATCAATT10508.0273335E-47.56363441
CCCCGAA8700.0097835197.46879241
CTCAAAA13101.4544664E-47.16471961
CTTGTTT15201.6139458E-57.1248351
CACATTT11200.00165659057.06503063
GATTTAC10300.00575647226.98396735
ATCAGAA14506.678288E-56.9708731
Produced by FastQC (version 0.11.5)