FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662609
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAT3200.006817652413.7088121
CTCTTGC4850.00845199110.5524861
CTCCCAT5600.00205620410.4448091
CTCCCGA5900.00317055389.9137171
CTTTCGA6150.00446778639.5107191
GATTTGG9201.948989E-48.6263835
CTTGAAA12004.53706E-68.5299271
AAGATAC10508.073035E-47.5583555
CTTTGTT12103.960863E-47.25094181
TTAAAGA11000.00132423487.21479378
CCTGTTT10450.00564857576.99652241
CTTATTT10750.0074301156.8012711
TTTAATT13400.00147069156.4619842
CTTCCTT13650.00156197596.42757461
CTTTTAT15308.265609E-46.2122721
Produced by FastQC (version 0.11.5)